The serological methods are primary methods in forensic investigations. Scientific methods using immunological reaction, chemical methods, the serological test methods are considered to be the most important to confirm the results of investigations. Basic element for any study is blood, and the science of bloodstain analysis traditionally follows certain steps which adequately describe the various tests conducted. Blood retrieved from stained cloth, stained earth samples, the weapons used etc., acquired from crime scene are to be collected for sampling. The origin of the sample whether it is of human or animal to be ascertained. Here, an effort is made to review the use of blood in serological tests on the biological materials available at the crime scene.