In the last two decades, Sanger sequencing has dominated genomics research and made significant advances, such as the sequencing of the entire human genome, which allowed for the discovery of single gene disorders and the detection of capable of attacking genetic abnormalities for clinical molecular diagnosis [1, 2]. There has been a dramatic increase in the sense and richness of genetic alterations in cancer, such as point mutations, small insertions or deletions, copy number changes, and structural variations, as a result of the application of next generation sequencing (NGS), particularly through whole genome (WGS) and whole excavation technology (WES).

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